ABSTRACT
O presente estudo avaliou a participação de agentes bacterianos e virais em abortos em bovinos de propriedades rurais do sul de Minas Gerais. Foi realizada análise histopatológica e imuno-histoquímica dos casos de aborto recebidos pelo Setor de Patologia Veterinária da Universidade Federal de Lavras no período de 1999 a 2013. De 60 fetos analisados, em 30 (50%) foram observadas lesões microscópicas. Destes, oito apresentavam lesões compatíveis com infecção por agentes bacterianos e três apresentaram lesões sugestivas de agentes virais. Dos abortos bacterianos, um feto tinha lesões compatíveis com leptospirose, caracterizadas por icterícia e colestase, nefrite intersticial linfoplasmocítica e nefrose tubular. Sete fetos apresentaram pneumonia ou broncopneumonia purulenta; num deles havia também pleurite e peritonite fibrinosas; e em dois desses fetos houve imunomarcação para Brucella abortus. Dos três fetos com lesões sugestivas de aborto viral ocorreu imunomarcação anti-Herpesvírus bovino em um. Os resultados demonstram a ocorrência de abortos de origem bacteriana e viral na Região do estudo e que medidas profiláticas devem ser adotadas nas propriedades. O trabalho demonstra também que a imuno-histoquímica (IHQ); associada à histopatologia; é uma ferramenta útil e viável para o diagnóstico, especialmente quando provas microbiológicas e/ou sorológicas não estão disponíveis.
The paper evaluates the participation of bacterial and viral agents in bovine abortions in farms of southern Minas Gerais state, Brazil. Histopathological and immunohistochemical analysis was performed in aborted fetuses of cattle received by the Setor de Patologia Veterinária, Universidade Federal de Lavras, from 1999 to 2013. From 60 fetuses studied, 30 (50%) had microscopic lesions. From these, eight had lesions consistent with bacterial agents and three had lesions suggestive of viral agents. In the bacterial abortions, one fetus presented lesions compatible with leptospirosis, characterized by jaundice, cholestasis, lymphoplasmacytic intersticial nephritis, and tubular nephrosis. Seven fetuses had purulent pneumonia or bronchopneumonia and one of them had also fibrinous pleuritis and peritonitis; two of them presented positive immunostaining for Brucella abortus. One of the three fetuses with lesions of viral infection revealed positive imunostaining for bovine herpesvirus. The results showed that abortions of bacterial and viral origin occur in the Region of this study and prophylactic measures should be adopted on the farms. This study also demonstrates that immunohistochemistry associated with histopathology is a useful and viable tool for the diagnosis, especially when microbiological and/or serological tests are not available.
Subject(s)
Animals , Female , Cattle , Abortion, Veterinary , Abortion, Septic/veterinary , Cattle/microbiology , Cattle/virology , Brucella abortus/isolation & purification , Aborted Fetus/anatomy & histology , Immunohistochemistry/veterinaryABSTRACT
Objective: The intent of this study was to evaluate the effect of plastination on the morphology and structure of stored organs, to find out how much accuracy a plastinated specimen has, and to look into the changes that occurred because of plastination. Materials and Methods: A human fetus of gestational age 24 weeks was plastinated, and 3D CT scan evaluation of the fetus was done. Results: The results showed normal, well-defined, clearly identifiable organs, with no alteration in morphology and structure of organs. Conclusion: In our opinion, plastinated specimens are better way of visualization of morphology and structure of stored organs, which is a useful tool for teaching as well as for research purposes.
Subject(s)
Aborted Fetus/anatomy & histology , Exhibitions as Topic , Health Education , Humans , Imaging, Three-Dimensional , Plastic Embedding/methods , Tomography, X-Ray ComputedABSTRACT
A Medicolegal case of embryo/foetus was received from the department of forensic medicine for the assessment of age of the foetus. The report for the assessment of age was prepared on the basis of gross appearance, radiological and histological examinations. On radiological examination of the limb tissue, the age of the foetus /embryo was approximately 3 months. The ossification centres for metatarsal bones were visible which appear at the 10th week of intrauterine life. Also on USG the femur length was found to be 10 mm which also suggests the age to be around 3 months. The smooth junction between the epidermis and dermis suggests age of the embryo/foetus to be less than 3 months because the epidermal ridges and dermal papillae become prominent at the end of 3rd month. From the basal layer of epidermis down growths were seen which were suggestive of growth of hair follicles. Hair follicles become prominent by the 10th week of intra uterine life which again reveals that the age of foetus is around end of 3rd month. From the observations, the age of the foetus was found to be more than 2 and half months but less than 3 months i.e. between 10-12 weeks.
Subject(s)
Aborted Fetus/anatomy & histology , Forensic Medicine/legislation & jurisprudence , Gestational Age/analysis , Humans , OsteogenesisABSTRACT
Relata-se o isolamento de Campylobacter jejuni de um feto ovino abortado no terço final da gestação. Em uma semana, observaram-se quatro casos de aborto em ovelhas com quatro meses de gestação. Imediatamente após o terceiro aborto, um dos fetos foi submetido a exame patológico, quando se realizou a colheita do conteúdo do abomaso para análise bacteriológica. A necropsia, somente foram evidenciadas alterações autolíticas. Apenas na microaerofilia encontrou-se crescimento bacteriano puro com características morfo-tintoriais de Campylobacter sp., identificado fenotípica e molecularmente como C. jejuni. Alerta-se para a possibilidade de quadros de abortos em ovinos serem causados por esse patógeno.